Mixing Performance Analysis of Orbitally Shaken Bioreactors
نویسندگان
چکیده
منابع مشابه
Hydrodynamic stress in orbitally shaken bioreactors
Materials and methods CHO-DG44 cells were cultivated in suspension in 1-L bottles as described in [1]. To determine conditions under which the shear stress was harmful for the cells, the bottles were orbitally shaken on an ES-X platform (Kühner AG, Birsfelden, Switzerland) at agitation rates from 150 to 200 rpm for 24 h. Control cultures were run in parallel with agitation at 110 rpm. The veloc...
متن کاملFluid dynamics and mixing behavior in orbitally shaken bioreactors for mammalian cell cultivation
Rationale and significance. Recombinant therapeutic proteins are frequently produced in mammalian cells cultivated in suspension in large-scale stirred tank bioreactors. There are three major drawbacks to stirred-tank bioreactors. First, they are expensive to purchase and maintain, limiting their availability to some potential users. Second, they have not been adapted to volumetric scales less ...
متن کاملkLa as a predictor for probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors
Background Orbitally shaken flasks are commonly used at an early stage of bioprocess development with mammalian cells. In contrast to large-scale stirred-tank bioreactors, shaken flasks are usually operated in probe-independent bioprocesses, i.e. without strictly controlling the pH or dissolved oxygen concentration (DO). As a consequence, gas transfer issues are thought to limit the effectivene...
متن کاملNumerical Simulation of Orbitally Shaken Reactors
Mammalian cell cultures have become a major topic of research in the biopharmaceutical industry. This kind of cells require specific conditions to grow. In this thesis, we study the hydrodynamics of orbitally shaken reactors (OSR), a recently introduced kind of bioreactors for mammalian cell cultures that represents a simple to operate and cheap alternative to commonly used reactors such as sti...
متن کاملShaken not Stirred: Mixing Semantics into XPDL
Ubiquitous computing requires lightweight approaches to coordinating tasks distributed across smart devices. We are currently developing a semantic workflow modelling approach that blends the proven robustness of XPDL with semantics to support proactive behaviour. We illustrate the potential of the model through an example based on mixing a dry martini.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied Sciences
سال: 2020
ISSN: 2076-3417
DOI: 10.3390/app10165597